Anticorps Polyclonal de lapin anti-MBP

MBP Polyclonal Antibody for IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-10458

Synonymes

myelin basic protein

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Galerie de données de validation

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  IF Staining of mouse brain using CL488-10458

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Myelin basic protein antibody (CL488-10458) at dilution of 1:200.

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  IF Staining of mouse brain using CL488-10458

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Myelin basic protein antibody (CL488-10458) at dilution of 1:200.

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  IF Staining of rat brain using CL488-10458

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite® Plus 488 MBP antibody (CL488-10458) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1, red), CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6, golden), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Cyan). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using CL488-10458

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite® Plus 488 MBP antibody (CL488-10458) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1, red), CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6, golden), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris, tissu cérébral de rat

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-10458 cible MBP dans les applications de IF-P et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MBP Protéine recombinante Ag0713
• Nom complet: myelin basic protein
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 14-23 kDa
• Numéro d’acquisition GenBank: BC008749
• Symbole du gène: Myelin basic protein
• Identification du gène (NCBI): 4155
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP.

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