Anticorps Monoclonal anti-MYH2-specific

MYH2-specific Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1F1B6

N° de cat : 66212-1-Ig

Synonymes

Myosin 2, MYH2, 1F1B6, MYHSA2, MyHC-IIa



Applications testées

Résultats positifs en WBtissu de muscle squelettique humain,
Résultats positifs en IHCtissu de muscle squelettique humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:100000
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66212-1-Ig cible MYH2-specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain, souris
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet myosin, heavy chain 2, skeletal muscle, adult
Masse moléculaire calculée 223 kDa
Poids moléculaire observé 200 kDa
Numéro d’acquisition GenBankNM_017534
Symbole du gène MYH2
Identification du gène (NCBI) 4620
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.

Protocole

Product Specific Protocols
WB protocol for MYH2-specific antibody 66212-1-IgDownload protocol
IHC protocol for MYH2-specific antibody 66212-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Agric Food Chem

Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.

Authors - Luchu Zhang
WB

Sci Rep

Trimetazidine and exercise provide comparable improvements to high fat diet-induced muscle dysfunction through enhancement of mitochondrial quality control.

Authors - Wenliang Zhang
mouseWB

J Muscle Res Cell Motil

RNA-seq and ChIP-seq unveils thyroid hormone receptor α deficiency affects skeletal muscle myoblast proliferation and differentiation via Col6a1 during aging

Authors - Runqing Shi
WB

Heliyon

Light-phase time-restricted feeding disrupts the muscle clock and insulin sensitivity yet potentially induces muscle fiber remodeling in mice

Authors - Zhou Ye
humanIHC

Sci Rep

Age-related thinning of orbicularis oculi muscle inside upper eyelid and its possible association with sunken upper eyelids

Authors - Takamasa Gomi
humanWB

J Biol Chem

O-GlcNAcylation modification of MyoD regulates skeletal muscle fiber differentiation by antagonizing the UPF1 pathway

Authors - Lele Kou
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