Anticorps Polyclonal de lapin anti-GLYR1

• Phare
• Validé par KD/KO

GLYR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 14833-1-AP

Synonymes

NDF, hNDF, HIBDL, Glyoxylate reductase 1 homolog, Cytokine-like nuclear factor N-PAC

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Galerie de données de validation

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  WB analysis using 14833-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14833-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14833-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 14833-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14833-1-AP

  IP result of anti-GLYR1 (IP:14833-1-AP, 4ug; Detection:14833-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IHC staining of mouse testis using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14833-1-AP (GLYR1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 14833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14833-1-AP (GLYR1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14833-1-AP

  Immunofluorescent analysis of Hela cells, using N-PAC antibody 14833-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du foie humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

14833-1-AP cible GLYR1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLYR1 Protéine recombinante Ag6637
• Nom complet: cytokine-like nuclear factor n-pac
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC064940
• Symbole du gène: N-PAC
• Identification du gène (NCBI): 84656
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human		Cell; Transcription factor protein interactomes reveal genetic determinants in heart disease.; Authors - Barbara Gonzalez-Teran
mouse	ChIP, WB	Genomics Proteomics Bioinformatics; Npac Is a Co-factor of Histone H3K36me3 and Regulates Transcriptional Elongation in Mouse Embryonic Stem Cells.; Authors - Sue Yu
human	WB,IHC,CoIP,IF	J Exp Clin Cancer Res; Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.; Authors - Zhiyan Hu ; KD Validated
mouse	WB	Int J Mol Sci; Npac Regulates Pre-mRNA Splicing in Mouse Embryonic Stem Cells; Authors - Yiwei Qian ; KO Validated

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