Anticorps Polyclonal de lapin anti-NAMPT/PBEF

• Phare
• Validé par KD/KO

NAMPT/PBEF Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

38

Avis (3)

N° de cat : 11776-1-AP

Synonymes

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

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Galerie de données de validation

• 

  WB analysis using 11776-1-AP

  Rat liver lysates were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 11776-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11776-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 11776-1-AP

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11776-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 11776-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11776-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 11776-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11776-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 11776-1-AP

  IP result of anti-NAMPT/PBEF (IP:11776-1-AP, 4ug; Detection:11776-1-AP 1:1000) with mouse heart tissue lysate 3720 ug.

• 

  IP experiment of mouse heart using 11776-1-AP

  IP result of anti-NAMPT/PBEF(IP:11776-1-AP, 4ug; Detection:11776-1-AP 1:4000) with mouse heart tissue lysate 1560 ug.

• 

  IHC staining of human lymphoma using 11776-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11776-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 11776-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of U-87 MG using 11776-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11776-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules HL-60, cellules Ramos, cellules RAW 264.7, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu cardiaque de souris,
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293, cellules HeLa, cellules MG U-87

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 35 publications below
• IHC: See 8 publications below
• IF: See 3 publications below

Informations sur le produit

11776-1-AP cible NAMPT/PBEF dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAMPT/PBEF Protéine recombinante Ag2434
• Nom complet: nicotinamide phosphoribosyltransferase
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC020691
• Symbole du gène: NAMPT
• Identification du gène (NCBI): 10135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Publications

Species	Application	Title
human	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala
human,mouse	IHC,WB	Cell Metab; NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.; Authors - Hongwei Lv ; KD Validated
mouse	WB,IHC	Cell Death Dis; Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy; Authors - Yang Deng
human,mouse	WB,IHC	Int J Mol Sci; NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.; Authors - Xue Li
human,mouse	IF,WB	Metabolism; Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.; Authors - Ya Li ; KD Validated
human	WB	Oncogenesis; Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma.; Authors - Anna E Gibson ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

DAN (Verified Customer) (03-11-2022)

Very effective in getting targeted efficacy.

• Applications: Western Blot
• Cell Tissue Type: Human, Mouse, Rat

FH

kishu (Verified Customer) (06-10-2021)

this Ab works well for WB (1:100) and IHC (1:200)

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: mouse liver tissues and rat liver

FH

Iru (Verified Customer) (05-30-2019)

This antibody works really well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: fallopian tube epithelial cells

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