Anticorps Polyclonal de lapin anti-NAP1L1

• Phare
• Validé par KD/KO

NAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 14898-1-AP

Synonymes

NAP1, NAP1L, NAP-1-related protein, NRP, Nucleosome assembly protein 1-like 1

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Galerie de données de validation

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  WB analysis using 14898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14898-1-AP

  WB result of NAP1L1 antibody (14898-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L1 transfected HeLa cells.

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  WB analysis of HT-1080 using 14898-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14898-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14898-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14898-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14898-1-AP

  IP result of anti-NAP1L1 (IP:14898-1-AP, 4ug; Detection:14898-1-AP 1:5000) with HEK-293 cells lysate 1440 ug.

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  IHC staining of human testis using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse testis using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14898-1-AP (NAP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 14898-1-AP (NAP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAP1L1 antibody (14898-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of NIH/3T3 using 14898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using NAP1L1 antibody (14898-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 14898-1-AP

  Immunofluorescent analysis of HepG2 cells, using NAP1L1 antibody 14898-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of NIH/3T3 using 14898-1-AP

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug NAP1L1 Polyclonal antibody (14898-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules A431, cellules HEK-293, cellules HeLa, cellules HT-1080, cellules Jurkat, tissu de thymus de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique de rat, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules NIH/3T3
• Résultats positifs en FC (Intra): cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

14898-1-AP cible NAP1L1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAP1L1 Protéine recombinante Ag6690
• Nom complet: nucleosome assembly protein 1-like 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC002387
• Symbole du gène: NAP1L1
• Identification du gène (NCBI): 4673
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Hum Reprod; Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage; Authors - Xiyao Wang
human	WB	Front Cell Dev Biol; NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.; Authors - Ye-Wei Zhang ; KD Validated
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
mouse	IF,WB	J Virol; NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.; Authors - Francisco Dominguez
human	WB,IHC,IF	Cancer Cell Int; NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.; Authors - Shu Liu ; KD Validated
human	WB	J Dermatol Sci; Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.; Authors - Satoru Mizuhashi

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