Anticorps Polyclonal de lapin anti-NAP1L1

• Phare
• Validé par KD/KO

NAP1L1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 14898-1-AP

Synonymes

hNRP, NAP 1 related protein, NAP1, NAP1L, NAP1L1, NRP

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Galerie de données de validation

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  WB analysis of Jurkat using 14898-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14898-1-AP

  WB result of NAP1L1 antibody (14898-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L1 transfected HeLa cells.

• 

  WB analysis of mouse thymus using 14898-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14898-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14898-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14898-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14898-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14898-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14898-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14898-1-AP

  IP Result of anti-NAP1L1 (IP:14898-1-AP, 4ug; Detection:14898-1-AP 1:1000) with HEK-293 cells lysate 800ug.

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  IHC staining of human testis using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 14898-1-AP

  Immunofluorescent analysis of MCF-7 cells, using NAP1L1 antibody 14898-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 14898-1-AP

  Immunofluorescent analysis of HepG2 cells, using NAP1L1 antibody 14898-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of MCF-7 using 14898-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug NAP1L1 antibody (14898-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules HEK-293, cellules HeLa, cellules HL-60, cellules HT-1080, tissu de thymus de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules HeLa
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

14898-1-AP cible NAP1L1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAP1L1 Protéine recombinante Ag6690
• Nom complet: nucleosome assembly protein 1-like 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC002387
• Symbole du gène: NAP1L1
• Identification du gène (NCBI): 4673
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.; Authors - Ye-Wei Zhang ; KD Validated
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
mouse	IF,WB	J Virol; NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.; Authors - Francisco Dominguez
human	WB,IHC,IF	Cancer Cell Int; NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.; Authors - Shu Liu ; KD Validated
human	WB	J Dermatol Sci; Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.; Authors - Satoru Mizuhashi
human	WB,IF	Cytoskeleton (Hoboken); NAP1L1 is a novel microtubule-associated protein; Authors - Nannan Peng