Anticorps Polyclonal de lapin anti-Napsin A

Napsin A Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 16558-1-AP

Synonymes

Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02

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Galerie de données de validation

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  WB analysis of mouse lung using 16558-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16558-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16558-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 16558-1-AP

  Immunofluorescent analysis of PC-3 cells using 16558-1-AP (Napsin A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16558-1-AP cible Napsin A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Napsin A Protéine recombinante Ag9786
• Nom complet: napsin A aspartic peptidase
• Masse moléculaire calculée: 420 aa, 45 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC017842
• Symbole du gène: NAPSA
• Identification du gène (NCBI): 9476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).