Anticorps Monoclonal anti-Napsin A

Napsin A Monoclonal Antibody for FC, IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1H7F2

N° de cat : 60259-2-Ig

Synonymes

Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02

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Galerie de données de validation

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  IHC staining of human lung cancer using 60259-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60259-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60259-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60259-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 60259-2-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 60259-2-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 60259-2-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (60259-2-Ig, Clone: 1H7F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HUVEC using 60259-2-Ig

  1X10^6 HUVEC cells were intracellularly stained with 0.25 ug Anti-Human Napsin A (60259-2-Ig, Clone:1H7F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using 60259-2-Ig

  1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human Napsin A (60259-2-Ig, Clone:1H7F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de carcinome à cellules rénales humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HUVEC,
• Résultats positifs en cytométrie: cellules A549, cellules HUVEC

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:10000-1:60000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

60259-2-Ig cible Napsin A dans les applications de IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Napsin A Protéine recombinante Ag9721
• Nom complet: napsin A aspartic peptidase
• Masse moléculaire calculée: 420 aa, 45 kDa
• Numéro d’acquisition GenBank: BC017842
• Symbole du gène: NAPSA
• Identification du gène (NCBI): 9476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

Publications

Species	Application	Title
human	IHC	Nat Commun; Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells.; Authors - Yanyun Du