Anticorps Polyclonal de lapin anti-NAS1/SLC13A1

NAS1/SLC13A1 Polyclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 25039-1-AP

Synonymes

SLC13A1, NAS1, hNaSi-1

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Galerie de données de validation

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  WB analysis of HEK-293 using 25039-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25039-1-AP (NAS1/SLC13A1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25039-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25039-1-AP (NAS1/SLC13A1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse kidney using 25039-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NAS1/SLC13A1 antibody (25039-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 25039-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NAS1/SLC13A1 antibody (25039-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal de souris
• Résultats positifs en IF-P: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25039-1-AP cible NAS1/SLC13A1 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAS1/SLC13A1 Protéine recombinante Ag19026
• Nom complet: solute carrier family 13 (sodium/sulfate symporters), member 1
• Masse moléculaire calculée: 595 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC148686
• Symbole du gène: NAS1
• Identification du gène (NCBI): 6561
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC13A1 also known as NAS1, is an apical membrane Na(+)-sulfate cotransporter involved in sulfate homeostasis in the kidney. SLC13A1 primarily participates in pH-independent, sodium-dependent cotransport of sulfate across renal proximal tubules and small intestinal epithelia(PMID: 38552027).

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