Anticorps Polyclonal de lapin anti-NAT1

• Phare
• Validé par KD/KO

NAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 19188-1-AP

Synonymes

Monomorphic arylamine N-acetyltransferase, MNAT, EC:2.3.1.5, Arylamine N-acetyltransferase 1, Arylamide acetylase 1

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Galerie de données de validation

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  WB analysis of PC-3 using 19188-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 19188-1-AP

  WB result of NAT1 antibody (19188-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT1 transfected PC-3 cells.

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  WB analysis of human placenta using 19188-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 19188-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 19188-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 19188-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 19188-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 19188-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 19188-1-AP

  Immunofluorescent analysis of Hela cells, using NAT1 antibody 19188-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 19188-1-AP

  Immunofluorescent analysis of HepG2 cells, using NAT1 antibody 19188-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules A375, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

19188-1-AP cible NAT1 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAT1 Protéine recombinante Ag6280
• Nom complet: N-acetyltransferase 1 (arylamine N-acetyltransferase)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 32-34 kDa
• Numéro d’acquisition GenBank: BC047666
• Symbole du gène: NAT1
• Identification du gène (NCBI): 9
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Front Oncol; Prognostic and Clinicopathological Significance of X-Box-Binding Protein 1 and N-Acetyltransferase 1 in Gallbladder Cancer.; Authors - Rushi Liu
human	WB,CoIP	Nucleic Acids Res; Integrated multi-omics approach revealed cellular senescence landscape; Authors - Qiao Song
Rat	WB	Int J Mol Sci; Hyperbaric Oxygen Therapy Alleviates Paclitaxel-Induced Peripheral Neuropathy Involving Suppressing TLR4-MyD88-NF-κB Signaling Pathway; Authors - Shih-Hung Wang

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