Anticorps Polyclonal de lapin anti-NAT10

• Phare
• Validé par KD/KO

NAT10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(72)

Avis (3)

N° de cat : 13365-1-AP

Synonymes

18S rRNA cytosine acetyltransferase, ALP, EC:2.3.1.-, KIAA1709, N acetyltransferase 10

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Galerie de données de validation

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  WB analysis of HeLa using 13365-1-AP

  WB result of NAT10 antibody (13365-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells.

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  WB analysis using 13365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13365-1-AP (NAT10 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13365-1-AP (NAT10 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13365-1-AP

  WB result of NAT10 antibody (13365-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected A549 cells.

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  IP experiment of HEK-293 using 13365-1-AP

  IP result of anti-NAT10 (IP:13365-1-AP, 4ug; Detection:13365-1-AP 1:30000) with HEK-293 cells lysate 1800 ug.

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  IP experiment of mouse testis using 13365-1-AP

  IP result of anti-NAT10 (IP:13365-1-AP, 4ug; Detection:13365-1-AP 1:8000) with mouse testis tissue lysate 1120 ug.

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  IHC staining of human ovary cancer using 13365-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13365-1-AP (NAT10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13365-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13365-1-AP (NAT10 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 13365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using NAT10 antibody (13365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HeLa using 13365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (13365-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of HeLa using 13365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (13365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules HEK-293, tissu testiculaire de souris
• Résultats positifs en IHC: human ovary cancer tissue, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 14 publications below
• WB: See 59 publications below
• IHC: See 10 publications below
• IF: See 14 publications below
• IP: See 4 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below
• RIP: See 8 publications below

Informations sur le produit

13365-1-AP cible NAT10 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, canin, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAT10 Protéine recombinante Ag4184
• Nom complet: N-acetyltransferase 10 (GCN5-related)
• Masse moléculaire calculée: 1025 aa, 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC035558
• Symbole du gène: NAT10
• Identification du gène (NCBI): 55226
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. NAT10 is the first enzyme to be found to catalyze ac4C production in eukaryotic RNA and has acetyltransferase activity and RNA-binding activity. (PMID: 23592795). NAT10 is the singular human enzyme to have both acetyltransferase and RNA binding activities (PMID: 30449621). NAT10 is associated with U3 small nucleolar RNA and acetylates upstream binding factors to activate rRNA transcription (PMID: 21177859). NAT10 promoted cisplatin chemoresistance in bladder cancer cells by enhancing DNA damage repair (DDR) (PMID: 36939377). NAT10 is a vital member of the Gcn5-related N-acetyltransferase (GNAT) family and contains 1,025 amino acids with a molecular weight of 116 kDa (PMID: 37128278). NAT10 is commonly expressed in lymphoid tissue, kidney, liver, cerebellum, cerebral cortex, and the central nervous system during the embryonic period. Normal tissue cells express NAT10 in the nucleus, while tumor cells translocate NAT10 to the cytoplasm, nucleoplasm, and nuclear membrane, affecting tumor formation (PMID: 37128278). The specificity of this antibody has been tested by siRNA. (24786082)

Publications

Species	Application	Title
human	WB,IP	Cell; Acetylation of Cytidine in mRNA Promotes Translation Efficiency.; Authors - Daniel Arango ; KO Validated
human	WB,IF	Science; Chemical inhibition of NAT10 corrects defects of laminopathic cells.; Authors - Delphine Larrieu ; KD Validated
human	WB,IP	Nat Commun; N-acetyltransferase 10 is implicated in the pathogenesis of cycling T cell-mediated autoimmune and inflammatory disorders in mice; Authors - Wen-Ping Li
human	WB	Cell Host Microbe; Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.; Authors - Kevin Tsai ; KO Validated
human	WB	Mol Cell; Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.; Authors - Daniel Arango
canine	WB	Nucleic Acids Res; N4-acetylcytidine coordinates with NP1 and CPSF5 to facilitate alternative RNA processing during the replication of minute virus of canines; Authors - Xueyan Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SONAM (Verified Customer) (12-18-2023)

Antibody works really well. No Nonspecific bindings.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cellls

FH

Mattia (Verified Customer) (09-12-2019)

Clear band at the right size, when antibody used at 1:1500 dilution in 5% milk an incubated at 2hrs at 25C. Band is a bit streaky, may require further optimisation.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: HeLa, HEK293T

FH

Jonathan (Verified Customer) (11-29-2018)

• Applications: Western Blot, Immunofluorescence,

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