Anticorps Polyclonal de lapin anti-NAT2

NAT2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 11410-1-AP

Synonymes

AAC2, Arylamide acetylase 2, Arylamine N-acetyltransferase 2, EC:2.3.1.118, EC:2.3.1.5

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Galerie de données de validation

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  WB analysis using 11410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11410-1-AP (NAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 11410-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 11410-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11410-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11410-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of MCF-7 using 11410-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug NAT2 Polyclonal antibody (11410-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat,
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

11410-1-AP cible NAT2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAT2 Protéine recombinante Ag1953
• Nom complet: N-acetyltransferase 2 (arylamine N-acetyltransferase)
• Masse moléculaire calculée: 33.5 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC015878
• Symbole du gène: NAT2
• Identification du gène (NCBI): 10
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NAT2 (Arylamine N-acetyltransferase 2) is well-known for its role in the phase II metabolism of xenobiotics and drugs (PMID:36156098). NAT2 and its isozyme, arylamine N-acetyltransferase 1 (NAT1), play key roles in the detoxification of carcinogenic arylamines and xenobiotics by catalyzing acetyl-coenzyme A (CoA)-dependent biotransformation of arylamines to N-arylacetamides. Endogenous NAT2 expression is limited to the liver and small and large intestines, suggesting that NAT2 plays organ-specific roles (PMID:24467436).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (04-26-2024)

Very good antibody for WB. Strong signal and clean backgroud.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse liver

NAT2 Antibody Western Blot validation (1:4000 dilution) in Mouse liver (Cat no:11410-1-AP)

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