Anticorps Polyclonal de lapin anti-NCAPG

• Phare
• Validé par KD/KO

NCAPG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 24563-1-AP

Synonymes

CAPG, CHCG, Chromosome-associated protein G, Condensin complex subunit 3, Condensin subunit CAP G

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Galerie de données de validation

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  WB analysis using 24563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24563-1-AP

  WB result of NCAPG antibody (24563-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPG transfected HEK-293 cells.

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  WB analysis using 24563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24563-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24563-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24563-1-AP

  IP result of anti-NCAPG (IP:24563-1-AP, 4ug; Detection:24563-1-AP 1:8000) with HEK-293 cells lysate 1800 ug.

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  IHC staining of human testis using 24563-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 24563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24563-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 24563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 24563-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NCAPG antibody (24563-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 24563-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NCAPG antibody (24563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

24563-1-AP cible NCAPG dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCAPG Protéine recombinante Ag19926
• Nom complet: non-SMC condensin I complex, subunit G
• Masse moléculaire calculée: 1015 aa, 114 kDa
• Poids moléculaire observé: 131 kDa
• Numéro d’acquisition GenBank: BC068467
• Symbole du gène: NCAPG
• Identification du gène (NCBI): 64151
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	J Transl Med; Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance; Authors - Kun Zheng
human	WB	Front Oncol; NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma.; Authors - Guangrong Zheng ; KD Validated
mouse,human	WB,IHC,CoIP	J Transl Med; NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN.; Authors - Rongguiyi Zhang ; KD Validated
human	WB	J Gastrointest Oncol; NCAPG is transcriptionally regulated by CBX3 and activates the Wnt/β-catenin signaling pathway to promote proliferation and the cell cycle and inhibit apoptosis in colorectal cancer; Authors - Hong Yang ; KD Validated
human	WB,IHC	Cell Death Dis; NCAPG confers trastuzumab resistance via activating SRC/STAT3 signaling pathway in HER2-positive breast cancer.; Authors - Lili Jiang ; KD Validated
human	IHC	Sci Rep; Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.; Authors - Yoshihiro Miyazaki

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