Anticorps Polyclonal de lapin anti-NCK1

• Phare
• Validé par KD/KO

NCK1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15247-1-AP

Synonymes

Cytoplasmic protein NCK1, NCK, Nck 1, NCK adaptor protein 1, NCK1, NCKalpha

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Galerie de données de validation

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  WB analysis of mouse brain using 15247-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15247-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15247-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15247-1-AP

  IP Result of anti-NCK1 (IP:15247-1-AP, 4ug; Detection:15247-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human breast cancer using 15247-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15247-1-AP (NCK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15247-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15247-1-AP (NCK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15247-1-AP

  Immunofluorescent analysis of Hela cells, using NCK1 antibody 15247-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15247-1-AP

  1X10^6 HeLa cells were stained with 0.2ug NCK1 antibody (15247-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15247-1-AP cible NCK1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCK1 Protéine recombinante Ag0979
• Nom complet: NCK adaptor protein 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC006403
• Symbole du gène: NCK1
• Identification du gène (NCBI): 4690
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NCK family adaptor proteins function to couple tyrosine phosphorylation signals to regulate actin cytoskeletal reorganization that leads to cell motility. Cytoplasmic protein NCK1 (or NCK alpha) is an adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 binds a number of intracellular proteins and influences various signaling pathways including GTPase-activating protein of Ras (RasGAP), which is responsible for the down-regulation of Ras. NCK1 is recently reported to regulate the UPR, which secondary to obesity impairs glucose homeostasis and INS actions.

Publications

Species	Application	Title
human	WB	Cell Signal; Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis.; Authors - Fan Zhang ; KD Validated
human	WB,IP	Mol Immunol; The adapter proteins ADAP and Nck cooperate in T cell adhesion.; Authors - Marcus Lettau ; KD Validated
human	WB,IHC	Mol Biol Rep; STAT3-induced NCK1 elevation promotes migration of triple-negative breast cancer cells via regulating ERK1/2 signaling; Authors - Peina He ; KD Validated
human	WB	Cell Microbiol; The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.; Authors - Lei He