Anticorps Polyclonal de lapin anti-NCL

• Phare
• Validé par KD/KO

NCL Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

33

Avis (1)

N° de cat : 10556-1-AP

Synonymes

C23, NCL, nucleolin, Protein C23

---

Galerie de données de validation

• 

  WB analysis using 10556-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10556-1-AP (NCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10556-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10556-1-AP (NCL Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IP experiment of HeLa using 10556-1-AP

  IP Result of anti-NCL (IP:10556-1-AP, 4ug; Detection:10556-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IHC staining of human prostate cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10556-1-AP (NCL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10556-1-AP (NCL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10556-1-AP (NCL Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10556-1-AP (NCL Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 10556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 10556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 10556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10556-1-AP

  1X10^6 HeLa cells were stained with 0.20ug NCL antibody (10556-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 12 publications below
• IP: See 3 publications below

Informations sur le produit

10556-1-AP cible NCL dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCL Protéine recombinante Ag0859
• Nom complet: nucleolin
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC006494
• Symbole du gène: NCL
• Identification du gène (NCBI): 4691
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

Publications

Species	Application	Title
human	WB	Nature; C9orf72 nucleotide repeat structures initiate molecular cascades of disease.; Authors - Aaron R Haeusler
rat	IF	Neuron; Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death.; Authors - Xinmei Wen
mouse	WB	Genome Res; The nuclear matrix protein HNRNPU maintains 3D genome architecture globally in mouse hepatocytes.; Authors - Hui Fan
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen ; KD Validated
mouse	WB	Protein Cell; Nuclear m6A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.; Authors - Chuan Chen
human,mouse	IF	EMBO J; The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.; Authors - Ella Doron-Mandel

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HEK293T for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

Produits associés

TOP1 Polyclonal antibody

20705-1-AP

B23/NPM1 Polyclonal antibody

10306-1-AP

human BCL2 Polyclonal antibody

12789-1-AP

P53 Polyclonal antibody

10442-1-AP

Glucocorticoid receptor Polyclonal antibody

24050-1-AP

PARP1 Polyclonal antibody

13371-1-AP