Anticorps Polyclonal de lapin anti-Kiz

Kiz Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 21177-1-AP

Synonymes

C20orf19, Centrosomal protein kizuna, HT013, KIZ, NCRNA00153, non protein coding RNA 153, PLK1S1, Polo like kinase 1 substrate 1

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Galerie de données de validation

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  WB analysis of HeLa using 21177-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21177-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21177-1-AP

  IP Result of anti-C20orf19 (IP:21177-1-AP, 3ug; Detection:21177-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human testis using 21177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 21177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 21177-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red).

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  IF Staining of hTERT-RPE1 using 21177-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

21177-1-AP cible Kiz dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kiz Protéine recombinante Ag15454
• Nom complet: non-protein coding RNA 153
• Masse moléculaire calculée: 673 aa, 75 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC045826
• Symbole du gène: NCRNA00153
• Identification du gène (NCBI): 55857
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (05-15-2022)

HEK293 cells stained for Hoechst (DNA marker, in green), Kizuna (in magenta) and PCNT (pericentriolar matrix marker, in green). HEK293 cells were plated on Poly-lysine coated coverslips and fixed in cold methanol for 5' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HEK293 cells (Human embryonic kidney 293 cells)

Kiz Antibody Immunofluorescence validation (1:200 dilution) in HEK293 cells (Human embryonic kidney 293 cells) (Cat no:21177-1-AP)