Anticorps Polyclonal de lapin anti-NDEL1

NDEL1 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17262-1-AP

Synonymes

EOPA, MITAP1, Mitosin associated protein 1, NDEL1, NUDEL, Protein Nudel

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Galerie de données de validation

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  WB analysis of human brain using 17262-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17262-1-AP (NDEL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17262-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17262-1-AP (NDEL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17262-1-AP

  IP Result of anti-NDEL1 (IP:17262-1-AP, 4ug; Detection:17262-1-AP 1:300) with HepG2 cells lysate 2400ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HepG2
• Résultats positifs en IP: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

17262-1-AP cible NDEL1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDEL1 Protéine recombinante Ag10975
• Nom complet: nudE nuclear distribution gene E homolog (A. nidulans)-like 1
• Masse moléculaire calculée: 345 aa, 38 kDa, 41 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC026101
• Symbole du gène: NDEL1
• Identification du gène (NCBI): 81565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear distribution factor E-homolog 1 (NDE1), NDE-like 1 (NDEL1), and Lissencephaly 1 (LIS1) together involve in essential neurodevelopmental processes, including neuronal precursor proliferation and differentiation, neurite outgrowth, and neuronal migration. All three bind directly to Disrupted in Schizophrenia 1 (DISC1), a scaffold protein critical to neuronal proliferation, integration, migration, and synaptic function within the developing and adult brain.[PMID: 18469341] Nuclear distribution element-like 1 (NDEL1) was firstly identified as a regulator of the cytoskeleton in microtubule and intermediate fliament dynamics and microtubule-based transport [PMID:21948775]. It is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome, which is in part by targeting the microtubule severing protein KATNA1 to the centrosome[PMID:15728732].

Publications

Species	Application	Title
mouse	WB,IF	J Cell Biol; Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis.; Authors - Sumigray Kaelyn D KD
mouse	IP, WB	Elife; Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis.; Authors - Youngsik Woo
human	WB,IF	Sci Rep; Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration.; Authors - Ji-Ho Hong
human	WB	Ann Neurol; Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.; Authors - Hyowon Hong
mouse	WB	Proc Natl Acad Sci U S A; Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment; Authors - Dong Jin Mun