Anticorps Polyclonal de lapin anti-NDNL2

NDNL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 27488-1-AP

Synonymes

HCA4, Hepatocellular carcinoma-associated protein 4, MAGEG1, MAGE-G1 antigen, Melanoma-associated antigen G1

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Galerie de données de validation

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  WB analysis of HepG2 using 27488-1-AP

  HepG2, L02 and HeLa cells were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 27488-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27488-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27488-1-AP

  HepG2 cells and human placenta tissue were subjected to SDS PAGE followed by western blot with 27488-1-AP (NDNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27488-1-AP (NDNL2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27488-1-AP (NDNL2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 27488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDNL2 antibody (27488-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules L02, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

27488-1-AP cible NDNL2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDNL2 Protéine recombinante Ag26663
• Nom complet: necdin-like 2
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC053999
• Symbole du gène: NDNL2
• Identification du gène (NCBI): 56160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDNL2 (also known as NSMCE3 or MAGEG1) is a part of the SMC5-6 protein complex that is essential for DNA damage response and chromosome segregation (PMID: 18086888; 27427983). The gene of human NDNL2 maps to chromosome 15q13.1. Northern blot analysis detected expression of a 1.9-kb transcript in all human tissues tested, with highest expression in testis (PMID: 11782285). Missense mutations in NSMCE3 have been associated with an autosomal recessive chromosome breakage syndrome that leads to defective T and B cell function and acute respiratory distress syndrome in early childhood (PMID: 27427983).

Publications

Species	Application	Title
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu

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