Anticorps Polyclonal de lapin anti-NDUFA5

NDUFA5 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16640-1-AP

Synonymes

B13, CI 13KD B, Complex I 13kD B, Complex I subunit B13, NDUFA5, NUFM, UQOR13

---

Galerie de données de validation

• 

  WB analysis of human brain using 16640-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16640-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16640-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16640-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 16640-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16640-1-AP (NDUFA5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16640-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16640-1-AP (NDUFA5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16640-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16640-1-AP (NDUFA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HeLa, tissu cardiaque humain, tissu hépatique de rat
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

16640-1-AP cible NDUFA5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFA5 Protéine recombinante Ag9995
• Nom complet: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC000813
• Symbole du gène: NDUFA5
• Identification du gène (NCBI): 4698
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFA5, also named as CI-13kD-B and NDUA5, belongs to the complex I NDUFA5 subunit family. It is accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which functions in the transfer of electrons from NADH to the respiratory chain. NDUFA5,an enrichment of mitochondrial protein, can be used as a mitochondrial marker protein(PMID:21360670).

Publications

Species	Application	Title
human	WB	EMBO Rep; MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.; Authors - Cong Wang
human	WB	Cancer Lett; PMID: 29906487; Authors - Linhua Lan
human	WB	J Mol Biol; COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria.; Authors - David Pacheu-Grau
human	WB	Proteomics; 2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to 14 consecutive days of endurance exercise training.; Authors - Egan Brendan B
human	WB	Evid Based Complement Alternat Med; Mechanistic Study of the Phytocompound, 2- β -D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches.; Authors - Jeng-Yuan Shiau
rat	WB	Mol Med Rep; Protein profiling and functional analysis of liver mitochondria from rats with nonalcoholic steatohepatitis.; Authors - Yanting You