Anticorps Polyclonal de lapin anti-NDUFS1

• Phare
• Validé par KD/KO

NDUFS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

67

N° de cat : 12444-1-AP

Synonymes

CI 75Kd, Complex I 75kD, NDUFS1, PRO1304

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Galerie de données de validation

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  WB analysis using 12444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12444-1-AP

  WB result of NDUFS1 antibody (12444-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS1 transfected HEK-293 cells.

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  WB analysis using 12444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12444-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12444-1-PBS

  A549 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12444-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12444-1-PBS

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12444-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 12444-1-AP

  IP Result of anti-NDUFS1 (IP:12444-1-AP, 4ug; Detection:12444-1-AP 1:500) with mouse lung tissue lysate 2800ug.

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  IHC staining of human liver cancer using 12444-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12444-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12444-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human liver cancer using 12444-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HepG2 using 12444-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 12444-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A549, cellules HEK-293, cellules HeLa, tissu hépatique de souris, tissu rénal de rat
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 64 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

12444-1-AP cible NDUFS1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Drosophile, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFS1 Protéine recombinante Ag3135
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 727 aa, 79 kDa
• Poids moléculaire observé: 74-81 kDa
• Numéro d’acquisition GenBank: BC030833
• Symbole du gène: NDUFS1
• Identification du gène (NCBI): 4719
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

Publications

Species	Application	Title
human	WB	Cell Discov; The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth; Authors - Xiaojun Ren
mouse	WB	Cell Metab; Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals; Authors - Timothy C Kenny
mouse	WB	Nat Med; Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I.; Authors - Chouchani Edward T ET
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
mouse	WB,IHC	Nat Neurosci; Engrailed protects mouse midbrain dopaminergic neurons against mitochondrial complex I insults.; Authors - Alvarez-Fischer Daniel D