Anticorps Polyclonal de lapin anti-NDUFS6

NDUFS6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 14417-1-AP

Synonymes

CI 13kD A, CI-13kD-A, Complex I 13kD A, Complex I-13kD-A, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

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Galerie de données de validation

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  WB analysis using 14417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14417-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 14417-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14417-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14417-1-AP

  IP result of anti-NDUFS6 (IP:14417-1-AP, 4ug; Detection:14417-1-AP 1:300) with mouse liver tissue lysate 3200 ug.

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  IHC staining of mouse brain using 14417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14417-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (14417-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 14417-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFS6 antibody (14417-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below

Informations sur le produit

14417-1-AP cible NDUFS6 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFS6 Protéine recombinante Ag5762
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC046155
• Symbole du gène: NDUFS6
• Identification du gène (NCBI): 4726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as 1CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

Publications

Species	Application	Title
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
human		Genes Dev; ALS/FTD-associated protein FUS induces mitochondrial dysfunction by preferentially sequestering respiratory chain complex mRNAs.; Authors - Yueh-Lin Tsai
mouse	WB	Theranostics; Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.; Authors - Lujing Jiang
human	WB	Cancer Lett; PMID: 29906487; Authors - Linhua Lan
mouse	WB	FASEB J; PMID: 31530015; Authors - Rufeng Zhang
mouse	WB	Front Aging Neurosci; Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.; Authors - Keqiang He

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