Anticorps Polyclonal de lapin anti-NDUFV3

NDUFV3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 13430-1-AP

Synonymes

Renal carcinoma antigen NY-REN-4, NADH-ubiquinone oxidoreductase 9 kDa subunit, Complex I-9kD, Complex I 9kD, CI-9kD

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Galerie de données de validation

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  WB analysis of HepG2 using 13430-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13430-1-AP (NDUFV3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13430-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13430-1-AP (NDUFV3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13430-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13430-1-AP (NDUFV3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13430-1-AP (NDUFV3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13430-1-AP (NDUFV3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13430-1-AP (NDUFV3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13430-1-AP (NDUFV3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 13430-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13430-1-AP (NDUFV3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cardiaque humain, tissu hépatique humain
• Résultats positifs en IHC: tissu placentaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

13430-1-AP cible NDUFV3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFV3 Protéine recombinante Ag4234
• Nom complet: NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa
• Masse moléculaire calculée: 108 aa, 12 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC033766
• Symbole du gène: NDUFV3
• Identification du gène (NCBI): 4731
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFV3(NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial) is also named as CI-9kD,Renal carcinoma antigen NY-REN-4 and belongs to the complex I NDUFV3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

Publications

Species	Application	Title
human	WB	J Proteome Res; Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone.; Authors - Villeneuve Lance L
human	WB	J Pharmacol Exp Ther; Metabolic Mechanisms and a Rational Combinational Application of Carboxyamidotriazole in Fighting Pancreatic Cancer Progression after Chemotherapy.; Authors - Rui Ju
human	WB	FEBS Lett; A novel isoform of the human mitochondrial complex I subunit NDUFV3.; Authors - Marris G Dibley
human	WB	F1000Res; Androgen-dependent alternative mRNA isoform expression in prostate cancer cells.; Authors - Jennifer Munkley
mouse	WB	Nat Struct Mol Biol; SCAF1 drives the compositional diversity of mammalian respirasomes; Authors - Irene Vercellino

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