Anticorps Polyclonal de lapin anti-NEDD4L

• Phare
• Validé par KD/KO

NEDD4L Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 13690-1-AP

Synonymes

hNedd4 2, KIAA0439, NEDD4 2, NEDD4.2, NEDD4L, NEDL3, RSP5

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Galerie de données de validation

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  WB analysis using 13690-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 13690-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 13690-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13690-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of C6 using 13690-1-AP

  IP result of anti-NEDD4L (IP:13690-1-AP, 4ug; Detection:13690-1-AP 1:300) with C6 cells lysate 1000 ug.

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  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13690-1-AP (NEDD4L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13690-1-AP (NEDD4L antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-3 using 13690-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (13690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules HT-1080, cellules PC-12, cellules PC-3
• Résultats positifs en IP: cellules C6,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IHC: See 9 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• CoIP: See 4 publications below

Informations sur le produit

13690-1-AP cible NEDD4L dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEDD4L Protéine recombinante Ag4565
• Nom complet: neural precursor cell expressed, developmentally down-regulated 4-like
• Masse moléculaire calculée: 854 aa, 96 kDa
• Poids moléculaire observé: 112 kDa, 125 kDa
• Numéro d’acquisition GenBank: BC032597
• Symbole du gène: NEDD4L
• Identification du gène (NCBI): 23327
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

Publications

Species	Application	Title
human	WB,IHC,IF	Nat Genet; Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia.; Authors - Loïc Broix
mouse	WB	J Exp Med; USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.; Authors - Siyuan Chen
rat	WB	Cell Death Differ; Aagab acts as a novel regulator of NEDD4-1-mediated Pten nuclear translocation to promote neurological recovery following hypoxic-ischemic brain damage.; Authors - Chunfang Dai
human	WB	Theranostics; JP1 suppresses proliferation and metastasis of melanoma through MEK1/2 mediated NEDD4L-SP1-Integrin αvβ3 signaling.; Authors - Jiahua Cui
rat,human	CoIP,IF	Pharmacol Res; Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2.; Authors - Lifang Huo
mouse	WB, IP, IF	Mol Cell Biol; Plk1 Phosphorylation of PTEN Causes a Tumor-Promoting Metabolic State.; Authors - Zhiguo Li