Anticorps Polyclonal de lapin anti-NEDD8

• Phare
• Validé par KD/KO

NEDD8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 16777-1-AP

Synonymes

cul nedd8, FLJ43224, Nedd 8, NEDD-8, Neddylin

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Galerie de données de validation

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  WB analysis of mouse brain using 16777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16777-1-AP (NEDD8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16777-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16777-1-AP (NEDD8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16777-1-AP (NEDD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16777-1-AP (NEDD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 16777-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16777-1-AP (NEDD8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 16777-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16777-1-AP (NEDD8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16777-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NEDD8 antibody (16777-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

16777-1-AP cible NEDD8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEDD8 Protéine recombinante Ag10154
• Nom complet: neural precursor cell expressed, developmentally down-regulated 8
• Masse moléculaire calculée: 81 aa, 9 kDa
• Poids moléculaire observé: 6-10 kDa
• Numéro d’acquisition GenBank: BC104664
• Symbole du gène: NEDD8
• Identification du gène (NCBI): 4738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEDD8 (neural precursor cell-expressed developmentally down-regulated gene 8) is a small ubiquitin-like protein that shares 60% sequence identity and 80% homology with ubiquitin [PMID:9353319]. NEDD8 is conjugated to substrate proteins in a process known as neddylation. NEDD8 forms a thioester bond with APPBP1-Uba3, the E1 enzyme. Activated NEDD8 then transfers to Ubc12, the E2 enzyme . Ultimately, E2 loaded with NEDD8 binds a substrate protein lysine residue and forms an isopeptide bond by E3 ligase [PMID:18802447].

Publications

Species	Application	Title
		Nat Neurosci; Identification of NUB1 as a suppressor of mutant Huntington toxicity via enhanced protein clearance.; Authors - Lu Boxun B
human	WB	J Transl Med; TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis; Authors - Xiang-Lin Hao
rat	WB,IF	Neuropsychopharmacology; Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.; Authors - Gonca Bayraktar ; KD Validated
		J Med Chem; Halo and Pseudohalo Gold(I)-NHC Complexes Derived from 4,5-Diarylimidazoles with Excellent in Vitro and in Vivo Anticancer Activity Against HCC.; Authors - Mianli Bian
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
human	IHC	J Exp Clin Cancer Res; Neddylation activated TRIM25 desensitizes triple-negative breast cancer to paclitaxel via TFEB-mediated autophagy; Authors - Bowen Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jose (Verified Customer) (09-14-2021)

Strong signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

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