Anticorps Polyclonal de lapin anti-NF-H/NF200

NF-H/NF200 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 21471-1-AP

Synonymes

200 kDa neurofilament protein, NEFH, Neurofilament H, NF, NF H, NF200, NFH, NF-H

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Galerie de données de validation

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  WB analysis of mouse brain tissue using 21471-1-AP

  WB result of 21471-1-AP.

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  WB analysis of mouse brain using 21471-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21471-1-AP (NF-H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21471-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21471-1-AP (NF-H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21471-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21471-1-AP (NF-H antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 21471-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21471-1-AP (NF-H antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 21471-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21471-1-AP (NF-H antibody at dilution of 1:200 (under 40x lens).

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  FC experiment of SH-SY5Y using 21471-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.5 ug Anti-Human NF-H (21471-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

21471-1-AP cible NF-H/NF200 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF-H/NF200 Protéine recombinante Ag15249
• Nom complet: neurofilament, heavy polypeptide
• Masse moléculaire calculée: 1026 aa, 112 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC073969
• Symbole du gène: NEFH
• Identification du gène (NCBI): 4744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd. This antibody recognize NEFH only.

Publications

Species	Application	Title
rat	WB,IF	Mater Today Bio; Ropivacaine microsphere-loaded electroconductive nerve dressings for long-acting analgesia and functional recovery following diabetic peripheral nerve injury; Authors - Fangguo Liang
mouse	WB	Sci Total Environ; Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.; Authors - Huanliang Liu
monkey	IF	iScience; Synthetic amyloid-β oligomers drive early pathological progression of Alzheimer's disease in nonhuman primates.; Authors - Feng Yue
rat		Toxicol Appl Pharmacol; Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.; Authors - Zhanjun Ma
mouse	IF	Plast Reconstr Surg Glob Open; A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model.; Authors - Jeffrey M Gimble

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