Anticorps Polyclonal de lapin anti-NF-L

NF-L Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 12998-1-AP

Synonymes

68 kDa neurofilament protein, CMT1F, CMT2E, NEFL, NF, NF L, NF68, NFL, NF-L

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Galerie de données de validation

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  WB analysis of mouse brain tissue using 12998-1-AP

  WB result of 12998-1-AP.

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  WB analysis using 12998-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12998-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 12998-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12998-1-AP

  IP Result of anti-NF-L (IP:12998-1-AP, 4ug; Detection:12998-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 12998-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NF-L antibody (12998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of mouse brain using 12998-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NF-L antibody (12998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 12998-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NF-L antibody (12998-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 12998-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NF-L (12998-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules C6, cellules Neuro-2a, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en cytométrie: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

12998-1-AP cible NF-L dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF-L Protéine recombinante Ag3667
• Nom complet: neurofilament, light polypeptide
• Masse moléculaire calculée: 543 aa, 62 kDa
• Poids moléculaire observé: 65-68 kDa, 140-160 kDa
• Numéro d’acquisition GenBank: BC039237
• Symbole du gène: NEFL
• Identification du gène (NCBI): 4747
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.; Authors - Zhengxin Ying
mouse	WB	Mater Today Bio; Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.; Authors - Luyu Wang
mouse	WB	Mater Today Bio; In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation.; Authors - Dan Zhang
rat	WB,IF	Adv Sci (Weinh); An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.; Authors - Jing Liu
		Front Mol Neurosci; Achaete-Scute Homolog 1 Expression Controls Cellular Differentiation of Neuroblastoma.; Authors - Mumtaz Kasim
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Max (Verified Customer) (02-24-2022)

strong and specific labeling of NfL in hamster brain lysate on western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hamster brain lysate

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