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Anticorps Monoclonal anti-NF-L

NF-L Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

5C12G4

N° de cat : 60189-1-Ig

Synonymes

68 kDa neurofilament protein, NEFL, Neurofilament L, NF, NF68, NFL, NF-L

Galerie de données de validation

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  • Western Blot (WB) analysis of various lysates using NF-L Monoclonal antibody (60189-1-Ig)

    WB analysis using 60189-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60189-1-Ig (NF-L antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IHC staining of rat brain using 60189-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IHC staining of mouse brain using 60189-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IHC staining of human brain using 60189-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IF Staining of mouse brain using 60189-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60189-1-Ig (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IF Staining of rat brain using 60189-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-L antibody (60189-1-Ig, Clone: 5C12G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of PC-12 cells using NF-L Monoclonal antibody (60189-1-Ig)

    FC experiment of PC-12 using 60189-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-L (60189-1-Ig, Clone:5C12G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en WBcellules PC-12, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
Résultats positifs en IHCtissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFtissu cérébral de souris, tissu cérébral de rat
Résultats positifs en cytométriecellules PC-12,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochimie (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

60189-1-Ig cible NF-L dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène NF-L Protéine recombinante Ag15178
Nom complet neurofilament, light polypeptide
Masse moléculaire calculée 543 aa, 62 kDa
Poids moléculaire observé 65 kDa
Numéro d’acquisition GenBankBC039237
Symbole du gène NEFL
Identification du gène (NCBI) 4747
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.

Protocole

Product Specific Protocols
WB protocol for NF-L antibody 60189-1-IgDownload protocol
IHC protocol for NF-L antibody 60189-1-IgDownload protocol
IF protocol for NF-L antibody 60189-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols