Anticorps Polyclonal de lapin anti-NEK1

• Phare
• Validé par KD/KO

NEK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 27146-1-AP

Synonymes

EC:2.7.11.1, KIAA1901, Never in mitosis A-related kinase 1, NimA related protein kinase 1, NimA-related protein kinase 1

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Galerie de données de validation

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  WB analysis of HeLa using 27146-1-AP

  WB result of NEK1 antibody (27146-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEK1 transfected HeLa cells.

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  WB analysis of PC-12 using 27146-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 27146-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-12 using 27146-1-AP

  IP result of anti-NEK1 (IP:27146-1-AP, 4ug; Detection:27146-1-AP 1:500) with PC-12 cells lysate 3080 ug.

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  IP experiment of HeLa using 27146-1-AP

  HeLa lysates prepared and IP of NEK1 performed using 1.0 μg of 27146-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human lung cancer using 27146-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27146-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27146-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NEK1 antibody (27146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules HeLa, tissu pancréatique de souris
• Résultats positifs en IP: cellules PC-12, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

27146-1-AP cible NEK1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEK1 Protéine recombinante Ag25970
• Nom complet: NIMA (never in mitosis gene a)-related kinase 1
• Masse moléculaire calculée: 1258 aa, 143 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: BC114491
• Symbole du gène: NEK1
• Identification du gène (NCBI): 4750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IP	Life Sci Alliance; Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases; Authors - Mateusz Gregorczyk
human	WB,IHC	Cancers (Basel); Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus; Authors - Lei Chen
human	WB	Adv Sci (Weinh); HDAC8 Enhances the Function of HIF-2α by Deacetylating ETS1 to Decrease the Sensitivity of TKIs in ccRCC; Authors - Kang Qian ; KD Validated

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