Anticorps Monoclonal anti-Neudesin/NENF

Neudesin/NENF Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

4G9E12

N° de cat : 60131-1-Ig

Synonymes

NENF, Neudesin, 4G9E12, Cell immortalization-related protein 2, Neuron-derived neurotrophic factor

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Galerie de données de validation

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  WB analysis using 60131-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 60131-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60131-1-Ig (NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cerebellum using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

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  IHC staining of human colon using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

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  IHC staining of human colon using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

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  IHC staining of human heart using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 60131-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Neudesin/NENF antibody (60131-1-Ig, Clone: 4G9E12 ) at dilution of 1:1900 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HepG2 using 60131-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Neudesin/NENF antibody (60131-1-Ig, Clone: 4G9E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HCT 116, cellules HepG2, cellules Jurkat, cellules LNCaP, tissu cérébral de porc, tissu cérébral humain
• Résultats positifs en IHC: tissu de cervelet humain, tissu cardiaque humain, tissu de cancer du côlon humain, tissu de côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

60131-1-Ig cible Neudesin/NENF dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Neudesin/NENF Protéine recombinante Ag8387
• Nom complet: neuron derived neurotrophic factor
• Masse moléculaire calculée: 172 aa, 19 kDa
• Poids moléculaire observé: 19 kDa, 16 kDa
• Numéro d’acquisition GenBank: BC008823
• Symbole du gène: NENF
• Identification du gène (NCBI): 29937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).

Publications

Species	Application	Title
human	WB	Cancer Cell Int; Identification of the tumor metastasis-related tumor subgroups overexpressed NENF in triple-negative breast cancer by single-cell transcriptomics; Authors - Guixin Wang

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