Anticorps Polyclonal de lapin anti-GNAS

• Phare
• Validé par KD/KO

GNAS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(17)

Avis (1)

N° de cat : 10150-2-AP

Synonymes

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

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Galerie de données de validation

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  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10150-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10150-2-AP

  IP result of anti-GNAS (IP:10150-2-AP, 4ug; Detection:10150-2-AP 1:5000) with mouse liver tissue lysate 2240 ug.

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  IHC staining of mouse brain using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10150-2-AP (GNAS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10150-2-AP (GNAS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10150-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GNAS antibody (10150-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594-phalloidine (red).

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  IF Staining of MCF-7 using 10150-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (10150-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de côlon humain, tissu cérébral de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

10150-2-AP cible GNAS dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNAS Protéine recombinante Ag0197
• Nom complet: GNAS complex locus
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC002722
• Symbole du gène: GNAS
• Identification du gène (NCBI): 2778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Publications

Species	Application	Title
human	WB,CoIP	Cancer Res; PRKDC Induces Chemoresistance in Osteosarcoma by Recruiting GDE2 to Stabilize GNAS and Activate AKT; Authors - Wenchao Zhang ; KD Validated
mouse	WB	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
human	WB	Cell Mol Life Sci; HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.; Authors - Gemoll Timo T
mouse	WB,IF	Sci Rep; Sex-specific transgenerational effects on murine thyroid gland imposed by ancestral exposure to neonicotinoid thiacloprid; Authors - Mariam Diba Lahmidi
human	WB,IF	Cell Mol Biol Lett; GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation.; Authors - Hongda Ding
mouse	WB,IF	Am J Physiol Renal Physiol; Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.; Authors - Lele Liu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (10-16-2023)

Works well.

• Applications: Western Blot

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