Anticorps Recombinant de lapin anti-NEU2

NEU2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240685C12

N° de cat : 83934-2-RR

Synonymes

SIAL2, N-acetyl-alpha-neuraminidase 2, N acetyl alpha neuraminidase 2, EC:3.2.1.18, Cytosolic sialidase

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Galerie de données de validation

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  IF Staining of PC-12 using 83934-2-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using NEU2 antibody (83934-2-RR, Clone: 240685C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 83934-2-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Neu2 Recombinant Antibody (83934-2-RR, Clone:240685C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules PC-12,
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83934-2-RR cible NEU2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NEU2 Protéine recombinante Ag17386
• Nom complet: sialidase 2 (cytosolic sialidase)
• Masse moléculaire calculée: 380 aa, 42 kDa
• Numéro d’acquisition GenBank: BC107053
• Symbole du gène: NEU2
• Identification du gène (NCBI): 4759
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEU2, also named as Sialidase 2, neuraminidase 2, catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides. NEU2 is localized in the cytosol and plasma membrane and takes part in myoblast and neuronal differentiation. It is almost undetectable under normal physiological conditions, and was found to be involved into apoptotic events in pancreatic ductal adenocarcinoma, one of the most dangerous cancers due to poor diagnosis and prognosis. (PMID: 30439363)

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