Anticorps Monoclonal anti-NEU3

• Phare
• Validé par KD/KO

NEU3 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2C9C5

N° de cat : 67098-1-Ig

Synonymes

cDNA FLJ78026, FLJ12388, NEU3, NEU3 protein, SIAL3

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Galerie de données de validation

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  WB analysis of PC-3 using 67098-1-Ig

  WB result of NEU3 antibody (67098-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEU3 transfected PC-3 cells.

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  WB analysis using 67098-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67098-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 67098-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67098-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67098-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67098-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67098-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules Caco-2, cellules COLO 320, cellules HT-29, cellules K-562, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67098-1-Ig cible NEU3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NEU3 Protéine recombinante Ag27272
• Nom complet: sialidase 3 (membrane sialidase)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48-52 kDa
• Numéro d’acquisition GenBank: NM_006656.5
• Symbole du gène: NEU3
• Identification du gène (NCBI): 10825
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.