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Anticorps Monoclonal anti-NF-L

NF-L Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

5C12G4

N° de cat : CL594-60189

Synonymes

68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, NF L, NF68, NFL, NF-L

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated NF-L Monoclonal antibody (CL594-60189)

    IF Staining of mouse brain using CL594-60189

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 NF-L antibody (CL594-60189, Clone: 5C12G4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite®594-conjugated NF-L Monoclonal antibody (CL594-60189)

    FC experiment of SH-SY5Y using CL594-60189

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NF-L (CL594-60189, Clone:5C12G4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFtissu cérébral de souris,
Résultats positifs en cytométriecellules SH-SY5Y

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60189 cible NF-L dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène NF-L Protéine recombinante Ag15178
Nom complet neurofilament, light polypeptide
Masse moléculaire calculée 543 aa, 62 kDa
Poids moléculaire observé 65 kDa
Numéro d’acquisition GenBankBC039237
Symbole du gène NEFL
Identification du gène (NCBI) 4747
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.

Protocole

Product Specific Protocols
IF protocol for CL594 NF-L antibody CL594-60189Download protocol
FC protocol for CL594 NF-L antibody CL594-60189Download protocol
Standard Protocols
Click here to view our Standard Protocols