Anticorps Monoclonal anti-NFATC2

NFATC2 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F3B2

N° de cat : 66917-1-Ig

Synonymes

NF ATc2, NF ATp, NFAT pre existing subunit, NFAT1, NFATC2, NFATP

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Galerie de données de validation

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  WB analysis using 66917-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66917-1-Ig (NFATC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66917-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66917-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66917-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66917-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 66917-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 66917-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 66917-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human NFATC2 (66917-1-Ig, Clone:1F3B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Ramos, cellules Daudi, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de lymphome humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66917-1-Ig cible NFATC2 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFATC2 Protéine recombinante Ag17990
• Nom complet: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
• Masse moléculaire calculée: 925 aa, 100 kDa
• Poids moléculaire observé: 135-140 kDa
• Numéro d’acquisition GenBank: BC136418
• Symbole du gène: NFATC2
• Identification du gène (NCBI): 4773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C