Anticorps Polyclonal de lapin anti-NFATC3

• Phare
• Validé par KD/KO

NFATC3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(32)

N° de cat : 18222-1-AP

Synonymes

NF AT4, NF ATc3, NFAT4, NF-AT4, NF-AT4c

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Galerie de données de validation

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  WB analysis using 18222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18222-1-AP (NFATC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18222-1-AP

  IP result of anti-NFATC3 (IP:18222-1-AP, 4ug; Detection:18222-1-AP 1:1000) with K-562 cells lysate 1760 ug.

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  IHC staining of mouse testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18222-1-AP (NFATC3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18222-1-AP (NFATC3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18222-1-AP (NFATC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18222-1-AP (NFATC3 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below

Informations sur le produit

18222-1-AP cible NFATC3 dans les applications de WB, IHC, IF, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFATC3 Protéine recombinante Ag12985
• Nom complet: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• Masse moléculaire calculée: 116 kDa
• Poids moléculaire observé: 130-170 kDa
• Numéro d’acquisition GenBank: BC001050
• Symbole du gène: NFATC3
• Identification du gène (NCBI): 4775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

Publications

Species	Application	Title
	ChIP	Immunity; Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.; Authors - Hongxu Xian
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
mouse	WB,IP	Cell Death Differ; Trim39 regulates neuronal apoptosis by acting as a SUMO-targeted E3 ubiquitin-ligase for the transcription factor NFATc3.; Authors - Meenakshi Basu-Shrivastava
human	WB	Oncogene; Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.; Authors - Mengyi Lao
human	WB	Cancer Lett; TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.; Authors - Da-Cen Lin
human	WB	Cell Biol Toxicol; HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells; Authors - Hanting Wang

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