Anticorps Polyclonal de lapin anti-NFE2

• Phare
• Validé par KD/KO

NFE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 11089-1-AP

Synonymes

p45 NF E2, p45, Nuclear factor, erythroid-derived 2 45 kDa subunit, NF E2, Leucine zipper protein NF-E2

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Galerie de données de validation

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11089-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11089-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11089-1-AP

  IP result of anti-NFE2 (IP:11089-1-AP, 4ug; Detection:11089-1-AP 1:500) with K-562 cells lysate 3440ug.

• 

  IHC staining of human colon cancer using 11089-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11089-1-AP (NFE2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11089-1-AP

  Immunofluorescent analysis of HepG2 cells, using NFE2 antibody 11089-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules U-937
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

11089-1-AP cible NFE2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFE2 Protéine recombinante Ag1570
• Nom complet: nuclear factor (erythroid-derived 2), 45kDa
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC005044
• Symbole du gène: NFE2
• Identification du gène (NCBI): 4778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. This is a rabbit polyclonal antibody raised against full-length NFE2 of human origin.

Publications

Species	Application	Title
human	WB	J Am Coll Cardiol; Heme levels are increased in human failing hearts.; Authors - Khechaduri Arineh A
mouse,human		Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human,mouse	WB,CoIP	Life Sci; Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys.; Authors - Shunying Jin ; KD Validated
human	WB	Life Sci; ERK and p38 MAPK inhibition controls NF-E2 degradation and profibrotic signaling in renal proximal tubule cells.; Authors - Jia Li
human	WB	BMC Genomics; Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages.; Authors - Carme Arnan
mouse	WB	Adv Sci (Weinh); Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.; Authors - Zhiyuan Lu ; KD Validated

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