Anticorps Monoclonal anti-NFE2

NFE2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1D7C8

N° de cat : 66436-1-Ig

Synonymes

1D7C8, Leucine zipper protein NF E2, Leucine zipper protein NF-E2, NF E2, Nuclear factor, erythroid-derived 2 45 kDa subunit

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Galerie de données de validation

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  WB analysis of HL-60 using 66436-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66436-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66436-1-Ig cible NFE2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFE2 Protéine recombinante Ag1570
• Nom complet: nuclear factor (erythroid-derived 2), 45kDa
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC005044
• Symbole du gène: NFE2
• Identification du gène (NCBI): 4778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

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