Anticorps Polyclonal de lapin anti-NFE2L1

• Phare
• Validé par KD/KO

NFE2L1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 12936-1-AP

Synonymes

HBZ17, LCR F1, Locus control region factor 1, NF E2 related factor 1, NFE2 related factor 1, NFE2L1, NRF1, TCF 11, TCF11, Transcription factor 11, Transcription factor HBZ17, Transcription factor LCR F1

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 12936-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12936-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12936-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12936-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12936-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12936-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12936-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Fetal mouse bone using 12936-1-AP

  Fetal mouse bone were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Fetal mouse bone using 12936-1-AP

  Fetal mouse bone were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12936-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12936-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12936-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12936-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 12936-1-AP

  IP Result of anti-NFE2L1 (IP:12936-1-AP, 4ug; Detection:12936-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human breast cancer using 12936-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12936-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, tissu osseux fœtal de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

12936-1-AP cible NFE2L1 dans les applications de WB, IP, IHC, IF, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFE2L1 Protéine recombinante Ag3916
• Nom complet: nuclear factor (erythroid-derived 2)-like 1
• Masse moléculaire calculée: 772 aa, 85 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC010623
• Symbole du gène: NFE2L1
• Identification du gène (NCBI): 4779
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580). This antibody is a rabbit polyclonal antibody raised against a region of human NFE2L1.

Publications

Species	Application	Title
human	WB,IF	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
mouse	WB,IP	Sci Adv; Lipid-anchored proteasomes control membrane protein homeostasis; Authors - Ruizhu Zhang
mouse	WB	Redox Biol; Long isoforms of NRF1 negatively regulate adipogenesis via suppression of PPARγ expression.; Authors - Peng Xue
mouse	WB	Free Radic Biol Med; Novel mechanistic insight on the neuroprotective effect of berberine: The role of PPARδ for antioxidant action.; Authors - Jia-Wen Shou
human	WB	Ecotoxicol Environ Saf; Long-isoform NFE2L1 silencing inhibits acquisition of malignant phenotypes induced by arsenite in human bronchial epithelial cells.; Authors - Yongfang Li ; KO Validated
mouse	WB	Food Chem Toxicol; Nfe2l1 deficiency mitigates streptozotocin-induced pancreatic β-cell destruction and development of diabetes in male mice; Authors - Simeng Bao