Anticorps Polyclonal de lapin anti-NRF2, NFE2L2

• Phare
• Validé par KD/KO

NRF2, NFE2L2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

1382

Avis (8)

N° de cat : 16396-1-AP

Synonymes

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2, NRF2, NFE2L2

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Galerie de données de validation

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  WB analysis using 16396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16396-1-AP

  DMSO treated HeLa cells were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16396-1-AP

  IP result of anti-NRF2, NFE2L2(IP:16396-1-AP, 4ug; Detection:16396-1-AP 1:6000) with HeLa cells lysate 1520 ug.

• 

  IHC staining of human pancreas cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16396-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16396-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (16396-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 16396-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRF2, NFE2L2 antibody (16396-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 16396-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug NRF2, NFE2L2 antibody (16396-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules HeLa traitées au DMSO
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu de cancer du sein humain, tissu de carcinome à cellules rénales humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris, cellules HepG2
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 132 publications below
• WB: See 1273 publications below
• IHC: See 170 publications below
• IF: See 317 publications below
• IP: See 25 publications below
• FC: See 1 publications below
• CoIP: See 16 publications below
• ChIP: See 14 publications below

Informations sur le produit

16396-1-AP cible NRF2, NFE2L2 dans les applications de WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, Lapin, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NRF2, NFE2L2 Protéine recombinante Ag9489
• Nom complet: nuclear factor (erythroid-derived 2)-like 2
• Masse moléculaire calculée: 605 aa, 68 kDa
• Poids moléculaire observé: 110 kDa, 68 kDa
• Numéro d’acquisition GenBank: BC011558
• Symbole du gène: NFE2L2
• Identification du gène (NCBI): 4780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Publications

Species	Application	Title
human,mouse	WB,IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human	WB	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
human	WB	Gastroenterology; Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis; Authors - Xiao-Mei Yang
human	WB, IP	Cancer Cell; iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.; Authors - Wenjie Ge ; KD Validated
human		Cell Metab; Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.; Authors - Jiajun Zhu
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guillermo (Verified Customer) (09-03-2022)

Very good product, great spots.

• Applications: Western Blot

FH

balawant (Verified Customer) (08-06-2022)

for westerblotting I have used this Antibody. this antibody is excellent and I will recommend if you are working with this protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: colon tissue and colon cancer cells

FH

Georgina (Verified Customer) (07-04-2022)

This is a good antibody - it gives some background signal but the band of interest is clear. Sometimes need to use a higher concentration than recommended for cells with lower expression of NRF2.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 500 or 1 in 1000
• Cell Tissue Type: Human HepG2 cells

FH

Iram (Verified Customer) (03-11-2022)

Gave good bands around 100 kda in colon cancer cell lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Colon cancer cells

FH

Giovanni (Verified Customer) (01-11-2022)

the antiboby worked very well at dilution 1:1000 in WB using Htr8/SVneo cell line lysates. It gave a specific band at around 100 kDa with very low background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Htr8/SVneo

NRF2, NFE2L2 Antibody Western Blot validation (1:1000 dilution) in Htr8/SVneo (Cat no:16396-1-AP)

FH

Xiaoping (Verified Customer) (10-14-2021)

Gave good bands around 100 kda in renal cell lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Kidney

FH

Razia Sultana (Verified Customer) (08-20-2020)

The antibody gives good band at around 100KD(100 KD in HK2 cells and 90KD in kidney tissue lysate) along with some non specific bands at around 40-50KD. The trial size gave a band at 130KD which is unusual and lot of non specific bands,but the full size antibody is good. We are satisfied with the purchase

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 in 5% milk
• Cell Tissue Type: Rat and mouse kidney tissue lysate and HK2 cell lysate

FH

JIM (Verified Customer) (03-26-2019)

Strong Nrf2 signal can be observed in macrophages treated with LPS and D3T by western blots.

• Applications: Western Blot,

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