Anticorps Recombinant de lapin anti-NFKB2,p100

• Phare
• Validé par KD/KO

NFKB2,p100 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241126C9

N° de cat : 84022-5-RR

Synonymes

NFKB2, 241126C9, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1

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Galerie de données de validation

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  WB analysis using 84022-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84022-5-RR (NFKB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 84022-5-RR

  WB result of NFKB2,p100 antibody (84022-5-RR; 1:60000; room temperature for 1.5 hours) with wild-type and sg-NFKB2 transfected HAP1 cells.

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  FC experiment of HeLa using 84022-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFKB2,p100 Recombinant antibody (84022-5-RR, Clone:241126C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A431 using 84022-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug NFKB2,p100 Recombinant antibody (84022-5-RR, Clone:241126C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84022-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84022-5-RR against Human NFKB2,p100 were performed. The affinity constant is 0.109 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HL-60, cellules Jurkat, cellules K-562, cellules SKOV-3
• Résultats positifs en FC (Intra): cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84022-5-RR cible NFKB2,p100 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NFKB2,p100 Protéine recombinante Ag0662
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC002844
• Symbole du gène: NFKB2
• Identification du gène (NCBI): 4791
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.

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