Anticorps Polyclonal de lapin anti-IkB Alpha

IkB Alpha Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 18220-1-AP

Synonymes

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

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Galerie de données de validation

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  WB analysis of HL-60 using 18220-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18220-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18220-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18220-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18220-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18220-1-AP (IkB alpha antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18220-1-AP

  IP Result of anti-IkB alpha (IP:18220-1-AP, 5ug; Detection:18220-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human kidney using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18220-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18220-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 18220-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 18220-1-AP (IkB alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18220-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18220-1-AP (IkB alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer de la prostate humain, tissu d'estomac humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below

Informations sur le produit

18220-1-AP cible IkB Alpha dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IkB Alpha Protéine recombinante Ag12979
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC004983
• Symbole du gène: NFKBIA
• Identification du gène (NCBI): 4792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publications

Species	Application	Title
human	WB	Mol Cancer; Activation of the NF-¿B pathway as a mechanism of alcohol enhanced progression and metastasis of human hepatocellular carcinoma.; Authors - Fei Wang
rat	WB	Front Mol Neurosci; Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats.; Authors - Jin Jiang
rat	WB	J Neuroinflammation; Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats.; Authors - Jian Zhan
mouse	WB	Biomed Pharmacother; Tubeimoside I improves survival of mice in sepsis by inhibiting inducible nitric oxide synthase expression.; Authors - Minghao Luo
human	WB	Cancer Biol Ther; MiR-376a-3p increases cell apoptosis in acute myeloid leukemia by targeting MT1X.; Authors - Xiangke Xin
mouse,rat	WB	Eur J Pharmacol; Tubeimoside I protects against sepsis-induced cardiac dysfunction via SIRT3.; Authors - Zhe Cheng