Anticorps Polyclonal de lapin anti-IkB Alpha

IkB Alpha Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 51066-1-AP

Synonymes

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

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Galerie de données de validation

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  WB analysis of PC-3 using 51066-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51066-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51066-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 51066-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 51066-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 51066-1-AP

  IP Result of anti-IkB alpha (IP:51066-1-AP, 4ug; Detection:51066-1-AP 1:1000) with HeLa cells lysate 3200ug.

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  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below

Informations sur le produit

51066-1-AP cible IkB Alpha dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC002601
• Symbole du gène: NFKBIA
• Identification du gène (NCBI): 4792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publications

Species	Application	Title
	WB	J Hazard Mater; Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and microbiota-independent manner.; Authors - Daiyang Xia
human	WB	J Immunol; Enterovirus 71 2C protein inhibits TNF-α-mediated activation of NF-κB by suppressing IκB kinase β phosphorylation.; Authors - Zheng Zhenhua Z
mouse	WB	Front Immunol; Aspartate Metabolism Facilitates IL-1β Production in Inflammatory Macrophages.; Authors - Hao Wang
mouse	WB	Front Immunol; Serine Supports IL-1β Production in Macrophages Through mTOR Signaling.; Authors - Siyuan Chen
mouse	WB	FASEB J; Nintedanib ameliorates osteoarthritis in mice by inhibiting synovial inflammation and fibrosis caused by M1 polarization of synovial macrophages via the MAPK/PI3K-AKT pathway; Authors - Jiangbo Yan
mouse	WB	Nutrients; Fish Hydrolysate Supplementation Containing n-3 Long Chain Polyunsaturated Fatty Acids and Peptides Prevents LPS-Induced Neuroinflammation.; Authors - Mathilde Chataigner