Anticorps Monoclonal anti-NHE1

• Phare
• Validé par KD/KO

NHE1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

3D9F3

N° de cat : 67363-1-Ig

Synonymes

SLC9A1, 3D9F3, APNH, APNH1, FLJ42224

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Galerie de données de validation

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  WB analysis using 67363-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67363-1-Ig (NHE1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules NCI-H1299, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Informations sur le produit

67363-1-Ig cible NHE1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NHE1 Protéine recombinante Ag14329
• Nom complet: solute carrier family 9 (sodium/hydrogen exchanger), member 1
• Masse moléculaire calculée: 815 aa, 91 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC012121
• Symbole du gène: NHE1
• Identification du gène (NCBI): 6548
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NHE1, also named as SLC9A1, is involved in pH regulation that can eliminate acids generated by active metabolism and counter adverse environmental conditions. NHE1 plays an important role in signal transduction. It has been shown that NHE1 participates in the development and progression of human breast cancer, gastric cancer and others. NHE1 has some isoforms with 91 kDa , 110 kDa (phosphoglycoprotein) and 210 kDa (dimeric protein). (PMID:28098891, 27751915, 8300588)

Publications

Species	Application	Title
rat	WB	Acta Pharmacol Sin; Direct cardio-protection of Dapagliflozin against obesity-related cardiomyopathy via NHE1/MAPK signaling.; Authors - Ke Lin ; KD Validated
human	IHC	Reproduction; An abnormal LPA/LPAR1-NHE1 axis leads to the autophagy deficiency of trophoblast cells in recurrent spontaneous abortion; Authors - Min Chen
human	WB	Cell Rep; Carbonic anhydrase 2 facilitates sorafenib resistance by counteracting MCT4-mediated intracellular pH dysregulation in HCC; Authors - Hui Lu
human	WB	Cell Rep; Mitochondrial transplantation rescues Ca2+ homeostasis imbalance and myocardial hypertrophy in SLC25A3-related hypertrophic cardiomyopathy; Authors - Shuang Li
human	WB	bioRxiv; Cystinosin is involved in Na+/H+ Exchanger 3 trafficking in the proximal tubular cells: new insights in the renal Fanconi syndrome in cystinosis; Authors - Veenita Khare
human	WB	Adv Sci (Weinh); A Human Engineered Heart Tissue-Derived Lipotoxic Diabetic Cardiomyopathy Model Revealed Early Benefits of Empagliflozin; Authors - Lin Cai

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