Anticorps Polyclonal de lapin anti-NHE3

NHE3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 27190-1-AP

Synonymes

SLC9A3, NHE-3

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Galerie de données de validation

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  WB analysis of mouse small intestine using 27190-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27190-1-AP (NHE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 27190-1-AP

  IP result of anti-NHE3 (IP:27190-1-AP, 4ug; Detection:27190-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse kidney using 27190-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH9). Arrows show brush border.

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  IHC staining of mouse kidney using 27190-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

27190-1-AP cible NHE3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NHE3 Protéine recombinante Ag25637
• Nom complet: solute carrier family 9 (sodium/hydrogen exchanger), member 3
• Masse moléculaire calculée: 834 aa, 93 kDa
• Poids moléculaire observé: 80-100 kDa
• Numéro d’acquisition GenBank: BC101669
• Symbole du gène: NHE3
• Identification du gène (NCBI): 6550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC9A3, also known as NHE3, belongs to the sodium hydrogen exchange protein family (NHE) and is mainly responsible for regulating the concentration of sodium and hydrogen ions inside and outside the cell. SLC9A3 is a Major apical Na+/H+ exchanger in kidney and intestine, playing an important role in renal and intestine Na+ absorption and blood pressure regulation (PMID:24622516, 2635877). Mutations in SLC9A3 cause Congenital Sodium Diarrhea (PMID: 26358773, 31276831).

Publications

Species	Application	Title
mouse	WB	Hypertension; Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.; Authors - Shuai Shao
mouse	WB	Hypertension; Podocyte Injury Augments Intrarenal Angiotensin II Generation and Sodium Retention in a Megalin-Dependent Manner.; Authors - Masahiro Koizumi
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen
pig	WB	J Virol; Pemetrexed alleviates piglet diarrhea by blocking the interaction between porcine epidemic diarrhea virus nucleocapsid protein and Ezrin; Authors - Shujuan Zhang
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang
pig	IHC	Front Vet Sci; Effects of Guava (Psidium guajava L.) Leaf Extract on the Metabolomics of Serum and Feces in Weaned Piglets Challenged by Escherichia coli.; Authors - Dingfa Wang

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