Anticorps Monoclonal anti-NINJ2

NINJ2 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

5C5B2

N° de cat : CL594-66202

Synonymes

Ninjurin-2, Nerve injury-induced protein 2, 5C5B2

---

Galerie de données de validation

• 

  FC experiment of U2OS using CL594-66202

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug Coralite®594 Ninj2 Monoclonal Antibody (CL594-66202, Clone:5C5B2)(red), or 0.4 ug CoraLite®594 Mouse IgG2b isotype control (11B8C4) (CL594-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66202 cible NINJ2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NINJ2 Protéine recombinante Ag5343
• Nom complet: ninjurin 2
• Masse moléculaire calculée: 142 aa, 16 kDa
• Numéro d’acquisition GenBank: BC057766
• Symbole du gène: NINJ2
• Identification du gène (NCBI): 4815
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NINJ2 (Ninjurin2) is a member of the ninjurin family of adhesion molecules which mediate cell-to-cell and cell-to-extracellular matrix interactions during development, differentiation, and regeneration of the peripheral nervous system. The gene encodes NINJ2 is located on chromosome 12p13. NINJ2 mRNA is widely expressed in adult human tissues, with highest level in bone marrow, followed by peripheral leukocytes, lung, and lymph nodes. In the peripheral nervous system, NINJ2 is expressed constitutively in mature sensory and enteric neurons. The expression of NINJ2 is upregulated after nerve injury in Schwann cells, suggesting that it may promote nerve regeneration. It may also play an important role in the pathogenesis of inflammatory disorder. (PMID: 10627596)

{{ptg:RelatedPrimaryAntibodies}}