Anticorps Monoclonal anti-NKRF

• Phare
• Validé par KD/KO

NKRF Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1H10C8

N° de cat : 68230-1-Ig

Synonymes

ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 68230-1-Ig

  WB result of NKRF antibody (68230-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKRF transfected HEK-293 cells.

• 

  WB analysis using 68230-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 68230-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

---

Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68230-1-Ig cible NKRF dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NKRF Protéine recombinante Ag6374
• Nom complet: NFKB repressing factor
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC068514
• Symbole du gène: NKRF
• Identification du gène (NCBI): 55922
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NKRF, also named as Protein ITBA4 or Transcription factor NRF, is a 690 amino acid protein, which contains one G-patch domain and one R3H domain. NKRF is Widely and constitutively expressed, with lower level in colon, peripheral blood lymphocytes, lung and kidney. NKRF interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. NKRF is involved in the constitutive silencing of the IFN-β promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity.