Anticorps Polyclonal de lapin anti-TTF1; NKX2-1

TTF1; NKX2-1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 21777-1-AP

Synonymes

BHC, Homeobox protein Nkx 2.1, NK 2, NK2 homeobox 1, NKX2 1, NKX2.1, NKX2-1, NKX2A, TEBP, Thyroid nuclear factor 1, Thyroid transcription factor 1, TITF1, TTF 1, TTF1, TTF1; NKX2-1

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Galerie de données de validation

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  WB analysis of HeLa using 21777-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21777-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21777-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21777-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21777-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

21777-1-AP cible TTF1; NKX2-1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: NK2 homeobox 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 41-45 kDa
• Numéro d’acquisition GenBank: NM_003317
• Symbole du gène: NKX2-1
• Identification du gène (NCBI): 7080
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NKX2-1, also named as NKX2A, TITF1 and TTF1, belongs to the NK-2 homeobox family. It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype. It may play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). The antibody is specific to NKX2-1.

Publications

Species	Application	Title
human	WB	Environ Toxicol Pharmacol; Role of estrogen receptors in thyroid toxicity induced by mono (2-ethylhexyl) phthalate via endoplasmic reticulum stress: An in vitro mechanistic investigation; Authors - Qi Xu