Anticorps Polyclonal de lapin anti-NLRX1

• Phare
• Validé par KD/KO

NLRX1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(24)

N° de cat : 17215-1-AP

Synonymes

NLR family member X1, NLRX 1, NOD5, NOD9, Nucleotide-binding oligomerization domain protein 5

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Galerie de données de validation

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  WB analysis using 17215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17215-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17215-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17215-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17215-1-AP

  IP result of anti-NLRX1 (IP:17215-1-AP, 3ug; Detection:17215-1-AP 1:800) with mouse heart tissue lysate 3520ug.

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  IHC staining of human heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

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  IF Staining of A549 using 17215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NLRX1 antibody (17215-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 17215-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NLRX1 (17215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, cellules THP-1, tissu cardiaque de rat, tissu cardiaque de souris, tissu de côlon de rat, tissu de côlon de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris, tissu de muscle squelettique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

17215-1-AP cible NLRX1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NLRX1 Protéine recombinante Ag11000
• Nom complet: NLR family member X1
• Masse moléculaire calculée: 975 aa, 108 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC013199
• Symbole du gène: NLRX1
• Identification du gène (NCBI): 79671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NLRX1 (Nucleotide-binding oligomerization domain, leucine-rich repeat containing X1) is a member of the NOD-like receptor (NLR) family and is unique among NLRs due to its localization to the mitochondrial matrix. NLRX1 is a negative regulator of innate immunity, particularly in viral infections. It interacts with the mitochondrial antiviral signaling protein (MAVS) to attenuate antiviral responses. NLRX1 has been implicated in various diseases, including multiple sclerosis, colorectal cancer, and ischemia-reperfusion injury. Its role in controlling inflammation and mitochondrial function makes it a potential therapeutic target.

Publications

Species	Application	Title
mouse	WB	Nature; Mitochondrial DNA stress primes the antiviral innate immune response.; Authors - A Phillip West
human	WB	Mol Cell; Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1.; Authors - Samuel A Killackey ; KD Validated
rat	WB,IF	Cell Death Differ; NLRX1 mediated impaired microglial phagocytosis of NETs in cerebral ischemia and reperfusion injury; Authors - Jialing Peng
human	WB,IHC	J Exp Med; NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity.; Authors - Geurt Stokman ; KO Validated
mouse,human	WB	PLoS Pathog; PMID: 28542569; Authors - Jae-Hoon Kim
human	WB	Front Immunol; PMID: 30344524; Authors - Tünde Fekete

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