Anticorps Polyclonal de lapin anti-NME1

NME1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (1)

N° de cat : 11086-2-AP

Synonymes

AWD, EC:2.7.4.6, GAAD, Granzyme A activated DNase, Granzyme A-activated DNase

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Galerie de données de validation

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  WB analysis of HeLa using 11086-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells using 11086-2-AP

  WB result of 10086-2-AP (NME1 antibody).

• 

  WB analysis of MCF-7 using 11086-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11086-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 11086-2-AP

  IP result of anti-NME1 (IP:11086-2-AP, 4ug; Detection:11086-2-AP 1:500) with A549 cells lysate 2800ug.

• 

  IHC staining of human liver using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11086-2-AP (NME1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11086-2-AP (NME1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 25x lens).

• 

  IF Staining of MCF-7 using 11086-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NME1 antibody (11086-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of A549 using 11086-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NME1 antibody (11086-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, multi-cellules
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11086-2-AP cible NME1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NME1 Protéine recombinante Ag1548
• Nom complet: non-metastatic cells 1, protein (NM23A) expressed in
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 16-20 kDa
• Numéro d’acquisition GenBank: BC018994
• Symbole du gène: NME1
• Identification du gène (NCBI): 4830
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination.It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
human	WB	Redox Biol; Regulation of metastasis suppressor NME1 by a key metabolic cofactor coenzyme A.; Authors - Bess Yi Kun Yu
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human	IF	J Transl Med; Identification and validation of smoking-related genes in lung adenocarcinoma using an in vitro carcinogenesis model and bioinformatics analysis.; Authors - Jin Wang
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
human	IF	Oncol Rep; LINC01816 promotes the migration, invasion and epithelial‑mesenchymal transition of thyroid carcinoma cells by sponging miR‑34c‑5p and regulating CRABP2 expression levels.; Authors - Hongyuan Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chantal (Verified Customer) (10-04-2022)

Specific band appeared after 1 minute of exposure. This antibody works on mouse and human cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MEFs, Hela, HEK293FT

NME1 Antibody Western Blot validation (1:1000 dilution) in MEFs, Hela, HEK293FT (Cat no:11086-2-AP)

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