Anticorps Recombinant de lapin anti-NME1/2

NME1/2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242698C7

N° de cat : 85074-2-RR

Synonymes

NME2, 242698C7, C-myc purine-binding transcription factor PUF, EC:2.7.13.3, EC:2.7.4.6

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Galerie de données de validation

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  WB analysis using 85074-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85074-2-RR (NME1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293T using 85074-2-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug NME1/2 Recombinant antibody (85074-2-RR, Clone:242698C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu hépatique de souris
• Résultats positifs en FC (Intra): cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85074-2-RR cible NME1/2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NME1/2 Protéine recombinante Ag13913
• Nom complet: non-metastatic cells 2, protein (NM23B) expressed in
• Masse moléculaire calculée: 152 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC002476
• Symbole du gène: NME2
• Identification du gène (NCBI): 4831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing. Refer to epitope and protein detection, the antibody 85074-2-RR detects both NME1& NME2.

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