Anticorps Polyclonal de lapin anti-NME3

• Phare
• Validé par KD/KO

NME3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 15136-1-AP

Synonymes

NDP kinase 3, NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23

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Galerie de données de validation

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  WB analysis using 15136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15136-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15136-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15136-1-AP

  IP result of anti-NME3 (IP:15136-1-AP, 4ug; Detection:15136-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

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  IHC staining of human breast cancer using 15136-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15136-1-AP (NME3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15136-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15136-1-AP (NME3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15136-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NME3 antibody (15136-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 15136-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 15136-1-AP (NME3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules K-562, cellules MCF-7, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15136-1-AP cible NME3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NME3 Protéine recombinante Ag7284
• Nom complet: non-metastatic cells 3, protein expressed in
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC000250
• Symbole du gène: NME3
• Identification du gène (NCBI): 4832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6).

Publications

Species	Application	Title
human,zebrafish	IF,WB	J Biol Chem; The nucleoside diphosphate kinase NME3 associates with nephronophthisis proteins, and is required for ciliary function during renal development.; Authors - Sylvia Hoff ; KD Validated
human	IHC	Onco Targets Ther; Exploring Protein Expression Profiles in Lung Cancer Insufficient Microwave Ablation: Implications for Recurrence; Authors - Peng Yan

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